Composite
Part:BBa_K4059014:Design
Designed by: Ann-Sofie Nielsen Group: iGEM21_SDU-Denmark (2021-10-21)
PsiH from Psilocybe cubensis with N-terminal substitution (4), E. coli K12 codon optimized
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 833
Illegal SapI.rc site found at 455
Design Notes
One of the chimera N-terminals which gave the best expression level from the referenced study
Source
https://pubmed.ncbi.nlm.nih.gov/23886957/